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Biomatters Ltd primer3 tool
Primer3 Tool, supplied by Biomatters Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer3+tool/primer3+tool/pmc12928595-39-39-46
Average 86 stars, based on 1 article reviews
primer3 tool - by Bioz Stars, 2026-11
86/100 stars

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Generated:

Article Title: Classification of Culturable Bifidobacterial Population from Colonic Samples of Wild Pigs (Sus scrofa) Based on Three Molecular Genetic Methods.
Article Snippet: Occurrence of bifidobacteria, known as healthpromoting probiotic microorganisms, in the digestive tract of wild pigs (Sus scrofa) has not been examined yet.. One hundred forty-nine fructose-6-phosphate phosphoketolase positive bacterial strains were isolated from colonic content of twenty-two individuals of wild pigs originated from four localities in the Czechia.. Based on PCR-DGGE technique targeting the variable V3 region of the 16S rRNA genes, strains were initially differentiated into four groups represented by: (i) probably a new Bifidobacterium species (89 strains), (ii) B. boum/B. thermophilum/B. thermacidophilum subsp. porcinum/B. thermacidophilum subsp. thermacidophilum (sub)species (49 strains), (iii) Pseudoscardovia suis (7 strains), and (iv) B. pseudolongum subsp. globosum/ B. pseudolongum subsp. pseudolongum (4 strains), respectively.

Software:

Article Title: Classification of Culturable Bifidobacterial Population from Colonic Samples of Wild Pigs (Sus scrofa) Based on Three Molecular Genetic Methods.
Article Snippet: Occurrence of bifidobacteria, known as healthpromoting probiotic microorganisms, in the digestive tract of wild pigs (Sus scrofa) has not been examined yet.. One hundred forty-nine fructose-6-phosphate phosphoketolase positive bacterial strains were isolated from colonic content of twenty-two individuals of wild pigs originated from four localities in the Czechia.. Based on PCR-DGGE technique targeting the variable V3 region of the 16S rRNA genes, strains were initially differentiated into four groups represented by: (i) probably a new Bifidobacterium species (89 strains), (ii) B. boum/B. thermophilum/B. thermacidophilum subsp. porcinum/B. thermacidophilum subsp. thermacidophilum (sub)species (49 strains), (iii) Pseudoscardovia suis (7 strains), and (iv) B. pseudolongum subsp. globosum/ B. pseudolongum subsp. pseudolongum (4 strains), respectively.

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : A potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary ) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Article Title: Iron metabolism modulation in Atlantic salmon infested with the sea lice Lepeophtheirus salmonis and Caligus rogercresseyi: A matter of nutritional immunity?
Article Snippet: Sea lice are copepodid ectoparasites that produce high economic losses and environmental issues, thus impacting the salmon aquaculture worldwide.. Atlantic salmon (Salmo salar) from Northern and Southern Hemispheres are primarily parasitized by Lepeophtheirus salmonis and Caligus rogercresseyi, respectively.. To cope L. salmonis infestation, studies suggest that Atlantic salmon can restrict iron availability as a mechanism of nutritional immunity.

Article Title: Increasing availability of reference mitochondrial genomes for imperiled fishes in western North America for environmental DNA assay design and species monitoring
Article Snippet: Primer design for all families and failed regions followed the methods of Schroeter et al. (2020). .. Briefly, this included identifying highly conserved regions among NCBI RefSeq (O'Leary et al., 2016) within family groups and designing primers in these regions using the Primer3 tool (Koressaar and Remm, 2007; Untergasser et al., 2012) in Geneious R11.1 software (Biomatters Ltd, Newark, NJ, USA). ..

Article Title: Identification and functional characterization of a pyrokinin neuropeptide receptor in the Lyme disease vector, Ixodes scapularis.
Article Snippet: Accepted Manuscript Title: Identification and functional characterization of a pyrokinin neuropeptide receptor in the Lyme disease vector, Ixodes scapularis Author: Kinsi Gondalia Anam Qudrat Brigida Bruno Janet Fleites Medina Jean-Paul V. Paluzzi PII: S0196-9781(16)30188-7 DOI: http://dx.doi.org/doi:10.1016/j.peptides.2016.09.011 Reference: PEP 69677 To appear in: Peptides Received date: 20-7-2016 Revised date: 19-9-2016 Accepted date: 21-9-2016 Please cite this article as: Gondalia Kinsi, Qudrat Anam, Bruno Brigida, Medina Janet Fleites, Paluzzi Jean-Paul V.Identification and functional characterization of a pyrokinin neuropeptide receptor in the Lyme disease vector, Ixodes scapularis.Peptides http://dx.doi.org/10.1016/j.peptides.2016.09.011 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : a potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary Table S1) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Sequencing:

Article Title: Classification of Culturable Bifidobacterial Population from Colonic Samples of Wild Pigs (Sus scrofa) Based on Three Molecular Genetic Methods.
Article Snippet: Occurrence of bifidobacteria, known as healthpromoting probiotic microorganisms, in the digestive tract of wild pigs (Sus scrofa) has not been examined yet.. One hundred forty-nine fructose-6-phosphate phosphoketolase positive bacterial strains were isolated from colonic content of twenty-two individuals of wild pigs originated from four localities in the Czechia.. Based on PCR-DGGE technique targeting the variable V3 region of the 16S rRNA genes, strains were initially differentiated into four groups represented by: (i) probably a new Bifidobacterium species (89 strains), (ii) B. boum/B. thermophilum/B. thermacidophilum subsp. porcinum/B. thermacidophilum subsp. thermacidophilum (sub)species (49 strains), (iii) Pseudoscardovia suis (7 strains), and (iv) B. pseudolongum subsp. globosum/ B. pseudolongum subsp. pseudolongum (4 strains), respectively.

other:

Article Title: Accumulation of cyanobacterial oxadiazine nocuolin A is enhanced by temperature shift during cultivation and is promoted by bacterial co-habitants in the culture
Article Snippet: Proper setting of cultivation conditions is essential for production of high-value compounds in microbial biotechnology.. The present study characterizes photoautotrophic growth and capacity to accumulate the antiproliferative secondary metabolite Nocuolin A (NoA) in cyanobacterium Nostoc sp. CCAP 1453/38.. As the cyanobacterial culture was found to be non-axenic, the bacteria accompanying the culture were characterized, then the growth demands and NoA production in the Nostoc-bacterial consortium were determined, and finally an axenic strain was prepared.

Article Title: Single nucleotide polymorphisms in cuticle and secretome-related genes are associated with hydrogen peroxide sensitivity in the sea louse Caligus rogercresseyi
Article Snippet: The sea louse Caligus rogercresseyi is the main ectoparasite species affecting the salmon industry in Chile.. Hydrogen peroxide (H2O2) is a chemotherapeutant frequently used for lice control, and currently considered as a non-pharmacological method.. The H2O2 molecules cause the formation of bubbles in the louse's hemolymph, inducing mechanical paralysis and detaching the parasite from the host.

Reverse Transcription:

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : A potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary ) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : a potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary Table S1) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Real-time Polymerase Chain Reaction:

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : A potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary ) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : a potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary Table S1) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Expressing:

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : A potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary ) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Article Title: Identification and functional characterization of a pyrokinin neuropeptide receptor in the Lyme disease vector, Ixodes scapularis.
Article Snippet: Accepted Manuscript Title: Identification and functional characterization of a pyrokinin neuropeptide receptor in the Lyme disease vector, Ixodes scapularis Author: Kinsi Gondalia Anam Qudrat Brigida Bruno Janet Fleites Medina Jean-Paul V. Paluzzi PII: S0196-9781(16)30188-7 DOI: http://dx.doi.org/doi:10.1016/j.peptides.2016.09.011 Reference: PEP 69677 To appear in: Peptides Received date: 20-7-2016 Revised date: 19-9-2016 Accepted date: 21-9-2016 Please cite this article as: Gondalia Kinsi, Qudrat Anam, Bruno Brigida, Medina Janet Fleites, Paluzzi Jean-Paul V.Identification and functional characterization of a pyrokinin neuropeptide receptor in the Lyme disease vector, Ixodes scapularis.Peptides http://dx.doi.org/10.1016/j.peptides.2016.09.011 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : a potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary Table S1) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Construct:

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : A potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary ) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..

Article Title: Characterization and deorphanization of RYamide signaling in Aedes aegypti : a potential regulator of hindgut-associated physiology
Article Snippet: .. Primers for reverse transcriptase quantitative PCR (RT-qPCR), synthesizing double-strand RNA (dsRNA) that targets the RYamide transcript, and mammalian expression constructs containing the two candidate RYamide receptors, specifically AAEL017005, NPYLR5 and AAEL019786, NPYLR7 (see Supplementary Table S1) were designed using the Primer3 tool in Geneious Pro Bioinformatics Software (Biomatters Ltd). ..



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